Development and validation of a sensitive ELISA for quantification of secretory IgA in rat saliva and faeces

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Development and validation of a sensitive ELISA for quantification of secretory IgA in rat saliva and faeces. / Hau, J.; Andersson, E.; Carlsson, H. E.

In: Laboratory Animals, Vol. 35, No. 4, 2001, p. 301-306.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hau, J, Andersson, E & Carlsson, HE 2001, 'Development and validation of a sensitive ELISA for quantification of secretory IgA in rat saliva and faeces', Laboratory Animals, vol. 35, no. 4, pp. 301-306. https://doi.org/10.1258/0023677011911822

APA

Hau, J., Andersson, E., & Carlsson, H. E. (2001). Development and validation of a sensitive ELISA for quantification of secretory IgA in rat saliva and faeces. Laboratory Animals, 35(4), 301-306. https://doi.org/10.1258/0023677011911822

Vancouver

Hau J, Andersson E, Carlsson HE. Development and validation of a sensitive ELISA for quantification of secretory IgA in rat saliva and faeces. Laboratory Animals. 2001;35(4):301-306. https://doi.org/10.1258/0023677011911822

Author

Hau, J. ; Andersson, E. ; Carlsson, H. E. / Development and validation of a sensitive ELISA for quantification of secretory IgA in rat saliva and faeces. In: Laboratory Animals. 2001 ; Vol. 35, No. 4. pp. 301-306.

Bibtex

@article{332ba7b29df04eec90abc0133b5a3326,
title = "Development and validation of a sensitive ELISA for quantification of secretory IgA in rat saliva and faeces",
abstract = "Non-invasive measures of immunological markers are an attractive means of stress assessment in laboratory animals. Salivary IgA has been used successfully as a stress marker in the human, and several reports indicate the potential of secretory IgA as a non-invasive measure of stress in animals. The present paper describes the development of an ELISA using commercially available components for the quantification of rat IgA and validation of this assay for the quantification of rat secretory IgA in saliva and faeces. The concentration of IgA in rat saliva varied significantly between duplicate samples obtained from individual rats, and the viscosity and small total volume of rat saliva gave unsatisfactory results for IgA. Faecal IgA was present in high concentrations, and duplicate samples varied by only 2-3%. However, faecal IgA seemed less stable than IgA in other biological compartments, and this finding must be taken into consideration when using quantitative measurements of IgA as a marker of mucous humoral immune status.",
keywords = "ELISA, Faeces, Mucosal immunity, Rat, Saliva, Secretory IgA, Stress assessment",
author = "J. Hau and E. Andersson and Carlsson, {H. E.}",
year = "2001",
doi = "10.1258/0023677011911822",
language = "English",
volume = "35",
pages = "301--306",
journal = "Laboratory Animals",
issn = "0023-6772",
publisher = "SAGE Publications",
number = "4",

}

RIS

TY - JOUR

T1 - Development and validation of a sensitive ELISA for quantification of secretory IgA in rat saliva and faeces

AU - Hau, J.

AU - Andersson, E.

AU - Carlsson, H. E.

PY - 2001

Y1 - 2001

N2 - Non-invasive measures of immunological markers are an attractive means of stress assessment in laboratory animals. Salivary IgA has been used successfully as a stress marker in the human, and several reports indicate the potential of secretory IgA as a non-invasive measure of stress in animals. The present paper describes the development of an ELISA using commercially available components for the quantification of rat IgA and validation of this assay for the quantification of rat secretory IgA in saliva and faeces. The concentration of IgA in rat saliva varied significantly between duplicate samples obtained from individual rats, and the viscosity and small total volume of rat saliva gave unsatisfactory results for IgA. Faecal IgA was present in high concentrations, and duplicate samples varied by only 2-3%. However, faecal IgA seemed less stable than IgA in other biological compartments, and this finding must be taken into consideration when using quantitative measurements of IgA as a marker of mucous humoral immune status.

AB - Non-invasive measures of immunological markers are an attractive means of stress assessment in laboratory animals. Salivary IgA has been used successfully as a stress marker in the human, and several reports indicate the potential of secretory IgA as a non-invasive measure of stress in animals. The present paper describes the development of an ELISA using commercially available components for the quantification of rat IgA and validation of this assay for the quantification of rat secretory IgA in saliva and faeces. The concentration of IgA in rat saliva varied significantly between duplicate samples obtained from individual rats, and the viscosity and small total volume of rat saliva gave unsatisfactory results for IgA. Faecal IgA was present in high concentrations, and duplicate samples varied by only 2-3%. However, faecal IgA seemed less stable than IgA in other biological compartments, and this finding must be taken into consideration when using quantitative measurements of IgA as a marker of mucous humoral immune status.

KW - ELISA

KW - Faeces

KW - Mucosal immunity

KW - Rat

KW - Saliva

KW - Secretory IgA

KW - Stress assessment

UR - http://www.scopus.com/inward/record.url?scp=0034811671&partnerID=8YFLogxK

U2 - 10.1258/0023677011911822

DO - 10.1258/0023677011911822

M3 - Journal article

C2 - 11669312

AN - SCOPUS:0034811671

VL - 35

SP - 301

EP - 306

JO - Laboratory Animals

JF - Laboratory Animals

SN - 0023-6772

IS - 4

ER -

ID: 369371420