Development of a saliva sampling method and a rocket immunoelectrophoretic assay for quantification of salivary IgA in the squirrel monkey (Saimiri sciureus)

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Development of a saliva sampling method and a rocket immunoelectrophoretic assay for quantification of salivary IgA in the squirrel monkey (Saimiri sciureus). / Carver, J. F.A.; Hau, J.

In: In Vivo, Vol. 14, No. 6, 2000, p. 735-739.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Carver, JFA & Hau, J 2000, 'Development of a saliva sampling method and a rocket immunoelectrophoretic assay for quantification of salivary IgA in the squirrel monkey (Saimiri sciureus)', In Vivo, vol. 14, no. 6, pp. 735-739.

APA

Carver, J. F. A., & Hau, J. (2000). Development of a saliva sampling method and a rocket immunoelectrophoretic assay for quantification of salivary IgA in the squirrel monkey (Saimiri sciureus). In Vivo, 14(6), 735-739.

Vancouver

Carver JFA, Hau J. Development of a saliva sampling method and a rocket immunoelectrophoretic assay for quantification of salivary IgA in the squirrel monkey (Saimiri sciureus). In Vivo. 2000;14(6):735-739.

Author

Carver, J. F.A. ; Hau, J. / Development of a saliva sampling method and a rocket immunoelectrophoretic assay for quantification of salivary IgA in the squirrel monkey (Saimiri sciureus). In: In Vivo. 2000 ; Vol. 14, No. 6. pp. 735-739.

Bibtex

@article{f497b4a75c124a67a1b60f1619589b8d,
title = "Development of a saliva sampling method and a rocket immunoelectrophoretic assay for quantification of salivary IgA in the squirrel monkey (Saimiri sciureus)",
abstract = "There has long been the requirement for a marker of well being and chronic stress that is, itself, unaffected by the transient stressors associated with sampling. Work done in the human, the rat and the dog suggests that salivary IgA may fulfil these criteria. This is the first report on the establishment of a saliva sampling technique and immunoelectrophoretic assay for quantification of secretory Immunoglobulin A (s-IgA) in the squirrel monkey (Saimiri sciureus). A safe and effective sampling method is described, as well as a detailed description of an accurate rocket immunoelectrophoretic assay developed for this study. Preliminary results demonstrate a large intra-individual variation, as well as inter-individual variation in s-IgA levels between successive days. This indicates that if stress is associated with decreased s-IgA levels in the squirrel monkey, the usefulness of s-IgA as a stress marker may be limited because analyses of large numbers of samples may be necessary in order to obtain significance.",
keywords = "Rocket immunoelectrophoresis, s-IgA, Saliva sampling, Salivary IgA, Stress",
author = "Carver, {J. F.A.} and J. Hau",
year = "2000",
language = "English",
volume = "14",
pages = "735--739",
journal = "In Vivo",
issn = "0258-851X",
publisher = "International Institute of Anticancer Research",
number = "6",

}

RIS

TY - JOUR

T1 - Development of a saliva sampling method and a rocket immunoelectrophoretic assay for quantification of salivary IgA in the squirrel monkey (Saimiri sciureus)

AU - Carver, J. F.A.

AU - Hau, J.

PY - 2000

Y1 - 2000

N2 - There has long been the requirement for a marker of well being and chronic stress that is, itself, unaffected by the transient stressors associated with sampling. Work done in the human, the rat and the dog suggests that salivary IgA may fulfil these criteria. This is the first report on the establishment of a saliva sampling technique and immunoelectrophoretic assay for quantification of secretory Immunoglobulin A (s-IgA) in the squirrel monkey (Saimiri sciureus). A safe and effective sampling method is described, as well as a detailed description of an accurate rocket immunoelectrophoretic assay developed for this study. Preliminary results demonstrate a large intra-individual variation, as well as inter-individual variation in s-IgA levels between successive days. This indicates that if stress is associated with decreased s-IgA levels in the squirrel monkey, the usefulness of s-IgA as a stress marker may be limited because analyses of large numbers of samples may be necessary in order to obtain significance.

AB - There has long been the requirement for a marker of well being and chronic stress that is, itself, unaffected by the transient stressors associated with sampling. Work done in the human, the rat and the dog suggests that salivary IgA may fulfil these criteria. This is the first report on the establishment of a saliva sampling technique and immunoelectrophoretic assay for quantification of secretory Immunoglobulin A (s-IgA) in the squirrel monkey (Saimiri sciureus). A safe and effective sampling method is described, as well as a detailed description of an accurate rocket immunoelectrophoretic assay developed for this study. Preliminary results demonstrate a large intra-individual variation, as well as inter-individual variation in s-IgA levels between successive days. This indicates that if stress is associated with decreased s-IgA levels in the squirrel monkey, the usefulness of s-IgA as a stress marker may be limited because analyses of large numbers of samples may be necessary in order to obtain significance.

KW - Rocket immunoelectrophoresis

KW - s-IgA

KW - Saliva sampling

KW - Salivary IgA

KW - Stress

UR - http://www.scopus.com/inward/record.url?scp=0034515210&partnerID=8YFLogxK

M3 - Journal article

C2 - 11204490

AN - SCOPUS:0034515210

VL - 14

SP - 735

EP - 739

JO - In Vivo

JF - In Vivo

SN - 0258-851X

IS - 6

ER -

ID: 369371831