Effects of Transdermal Fentanyl Treatment on Acute Pain and Inflammation in Rats with Adjuvant-induced Monoarthritis
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Effects of Transdermal Fentanyl Treatment on Acute Pain and Inflammation in Rats with Adjuvant-induced Monoarthritis. / Berke, Mie S; Colding-Jørgensen, Pernille; Pedersen, Line G.; Hestehave, Sara; Kalliokoski, Otto; Jensen, Henrik E; Sørensen, Dorte B; Hau, Jann; Abelson, Klas Sp.
In: Comparative Medicine, Vol. 72, No. 5, 2022, p. 320–329.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Effects of Transdermal Fentanyl Treatment on Acute Pain and Inflammation in Rats with Adjuvant-induced Monoarthritis
AU - Berke, Mie S
AU - Colding-Jørgensen, Pernille
AU - Pedersen, Line G.
AU - Hestehave, Sara
AU - Kalliokoski, Otto
AU - Jensen, Henrik E
AU - Sørensen, Dorte B
AU - Hau, Jann
AU - Abelson, Klas Sp
N1 - Publisher Copyright: Copyright 2022 by the American Association for Laboratory Animal Science.
PY - 2022
Y1 - 2022
N2 - Eliminating unnecessary pain is an important requirement of performing animal experimentation, including reducing and controlling pain of animals used in pain research. The goal of this study was to refine an adjuvant-induced monoarthritis model in rats by providing analgesia with a transdermal fentanyl solution (TFS). Male and female Sprague-Dawley rats, single- or pair-housed, were injected with 20 μL of complete Freund adjuvant (CFA) into the left ankle joint. CFA-injected rats treated with a single dose of transdermal fentanyl solution (0.33 or 1 mg/kg) were compared with an untreated CFA-injected group and sham groups that received either no treatment or TFS treatment (1 mg/kg) during 72 h. At the tested doses, TFS reduced mechanical hyperalgesia and improved the mobility, stance, rearing, and lameness scores at 6 h after CFA injection. Joint circumferences were not reduced by TFS treatment, and no significant differences were detected between the 2 doses of TFS, or between single- and pair-housed rats. Treatment with TFS did not appear to interfere with model development and characteristics. However, overall, the analgesic effect was transient, and several opioid-related side effects were observed.
AB - Eliminating unnecessary pain is an important requirement of performing animal experimentation, including reducing and controlling pain of animals used in pain research. The goal of this study was to refine an adjuvant-induced monoarthritis model in rats by providing analgesia with a transdermal fentanyl solution (TFS). Male and female Sprague-Dawley rats, single- or pair-housed, were injected with 20 μL of complete Freund adjuvant (CFA) into the left ankle joint. CFA-injected rats treated with a single dose of transdermal fentanyl solution (0.33 or 1 mg/kg) were compared with an untreated CFA-injected group and sham groups that received either no treatment or TFS treatment (1 mg/kg) during 72 h. At the tested doses, TFS reduced mechanical hyperalgesia and improved the mobility, stance, rearing, and lameness scores at 6 h after CFA injection. Joint circumferences were not reduced by TFS treatment, and no significant differences were detected between the 2 doses of TFS, or between single- and pair-housed rats. Treatment with TFS did not appear to interfere with model development and characteristics. However, overall, the analgesic effect was transient, and several opioid-related side effects were observed.
U2 - 10.30802/AALAS-CM-21-000066
DO - 10.30802/AALAS-CM-21-000066
M3 - Journal article
C2 - 36229169
VL - 72
SP - 320
EP - 329
JO - Comparative Medicine
JF - Comparative Medicine
SN - 1532-0820
IS - 5
ER -
ID: 322871476