High plasma corticosterone levels persist during frequent automatic blood sampling in rats
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High plasma corticosterone levels persist during frequent automatic blood sampling in rats. / Abelson, Klas S P; Adem, Bashir; Royo, Felix; Carlsson, Hans-Erik; Hau, Jann.
In: In Vivo, Vol. 19, No. 5, 2005, p. 815-9.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - High plasma corticosterone levels persist during frequent automatic blood sampling in rats
AU - Abelson, Klas S P
AU - Adem, Bashir
AU - Royo, Felix
AU - Carlsson, Hans-Erik
AU - Hau, Jann
PY - 2005
Y1 - 2005
N2 - Corticosterone levels in blood may be used as a marker of stress in rodents, provided that the blood sampling procedure itself is non-stressful. Automated blood sampling equipment (Accusampler) allows blood sampling without any interference with the animal and might be useful as a tool for an on-line measurement of stress markers in blood. However, the impact of the blood sampling itself on the corticosterone levels in blood is unknown. The present study was designed to evaluate whether the frequency of blood sampling influences the plasma corticosterone levels in male and female rats. During anaesthesia, a catheter was placed in the jugular vein and attached to an Accusampler. Blood samples (200 microl) were withdrawn with a high (24 samples) or low frequency (3 samples) during a six-hour period immediately after the catheter insertion. The corticosterone levels in the plasma were quantified with ELISA. The corticosterone levels persisted at high post-operation concentrations when blood was collected frequently, while the levels steadily declined significantly during low-frequency sampling. The corticosterone levels were higher in female than in male rats, but the curves were similar. The present study elucidates the importance of considering the frequency of blood withdrawal during automated blood sampling. This parameter may have an impact on the experimental results when using blood corticosterone levels as a stress marker, but also during any in vivo study where blood is collected, since high corticosterone levels may affect the normal physiology of the animals.
AB - Corticosterone levels in blood may be used as a marker of stress in rodents, provided that the blood sampling procedure itself is non-stressful. Automated blood sampling equipment (Accusampler) allows blood sampling without any interference with the animal and might be useful as a tool for an on-line measurement of stress markers in blood. However, the impact of the blood sampling itself on the corticosterone levels in blood is unknown. The present study was designed to evaluate whether the frequency of blood sampling influences the plasma corticosterone levels in male and female rats. During anaesthesia, a catheter was placed in the jugular vein and attached to an Accusampler. Blood samples (200 microl) were withdrawn with a high (24 samples) or low frequency (3 samples) during a six-hour period immediately after the catheter insertion. The corticosterone levels in the plasma were quantified with ELISA. The corticosterone levels persisted at high post-operation concentrations when blood was collected frequently, while the levels steadily declined significantly during low-frequency sampling. The corticosterone levels were higher in female than in male rats, but the curves were similar. The present study elucidates the importance of considering the frequency of blood withdrawal during automated blood sampling. This parameter may have an impact on the experimental results when using blood corticosterone levels as a stress marker, but also during any in vivo study where blood is collected, since high corticosterone levels may affect the normal physiology of the animals.
KW - Anesthesia
KW - Animals
KW - Automation
KW - Blood Specimen Collection
KW - Catheterization
KW - Catheters, Indwelling
KW - Corticosterone
KW - Enzyme-Linked Immunosorbent Assay
KW - Female
KW - Jugular Veins
KW - Male
KW - Rats
KW - Rats, Sprague-Dawley
KW - Sex Factors
KW - Stress, Physiological
M3 - Journal article
C2 - 16097432
VL - 19
SP - 815
EP - 819
JO - In Vivo
JF - In Vivo
SN - 0258-851X
IS - 5
ER -
ID: 48010454