Oral immunisation of chickens using cholera toxin B subunit and softigenR as adjuvants results in high antibody titre in the egg yolk

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Oral immunisation of chickens using cholera toxin B subunit and softigenR as adjuvants results in high antibody titre in the egg yolk. / Hedlund, G. P.; Hau, J.

In: In Vivo, Vol. 15, No. 5, 2001, p. 381-384.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hedlund, GP & Hau, J 2001, 'Oral immunisation of chickens using cholera toxin B subunit and softigenR as adjuvants results in high antibody titre in the egg yolk', In Vivo, vol. 15, no. 5, pp. 381-384.

APA

Hedlund, G. P., & Hau, J. (2001). Oral immunisation of chickens using cholera toxin B subunit and softigenR as adjuvants results in high antibody titre in the egg yolk. In Vivo, 15(5), 381-384.

Vancouver

Hedlund GP, Hau J. Oral immunisation of chickens using cholera toxin B subunit and softigenR as adjuvants results in high antibody titre in the egg yolk. In Vivo. 2001;15(5):381-384.

Author

Hedlund, G. P. ; Hau, J. / Oral immunisation of chickens using cholera toxin B subunit and softigenR as adjuvants results in high antibody titre in the egg yolk. In: In Vivo. 2001 ; Vol. 15, No. 5. pp. 381-384.

Bibtex

@article{4971658565a741cea623ab26793d84b2,
title = "Oral immunisation of chickens using cholera toxin B subunit and softigenR as adjuvants results in high antibody titre in the egg yolk",
abstract = "Oral immunisation by gavage of laying hens with human immunoglobulin G (IgG) combined with a number of potential adjuvants was performed. The resulting immuno-specific egg yolk (IgY) antibodies were quantified by ELISA. The following adjuvants were tested: A Poly(lactide-co-glycolide) (PLG) microspheres, Cholera toxin B-subunit (CTB), CTB conjugated with glutaraldehyde, Dimethyl dioctadecyl ammonium bromide (DDA), and Softigen{\textregistered} (pegylated C8/C10 mono/di glyceride). Hens in a positive control group were immunised with human IgG in saline emulsified with an equal volume of Freund's Incomplete Adjuvant. High titres of immunospecific IgY antibodies against human IgG were recorded in the eggs from the chickens immunised orally with the antigen combined with glutaraldehyde conjugated CTB and in the chickens immunised with the antigen combined with Softigen{\textregistered}. The present results show that invasive technique related stress could be eliminated/reduced in polyclonal antibody producing animals.",
keywords = "Animal welfare, Chicken, Cholera toxin, Egg yolk antibody, Hen, IgY, Immunoglobulin Y, Oral immunisation, Pegylated C8/C10 mono/diglyceride, Softigen",
author = "Hedlund, {G. P.} and J. Hau",
year = "2001",
language = "English",
volume = "15",
pages = "381--384",
journal = "In Vivo",
issn = "0258-851X",
publisher = "International Institute of Anticancer Research",
number = "5",

}

RIS

TY - JOUR

T1 - Oral immunisation of chickens using cholera toxin B subunit and softigenR as adjuvants results in high antibody titre in the egg yolk

AU - Hedlund, G. P.

AU - Hau, J.

PY - 2001

Y1 - 2001

N2 - Oral immunisation by gavage of laying hens with human immunoglobulin G (IgG) combined with a number of potential adjuvants was performed. The resulting immuno-specific egg yolk (IgY) antibodies were quantified by ELISA. The following adjuvants were tested: A Poly(lactide-co-glycolide) (PLG) microspheres, Cholera toxin B-subunit (CTB), CTB conjugated with glutaraldehyde, Dimethyl dioctadecyl ammonium bromide (DDA), and Softigen® (pegylated C8/C10 mono/di glyceride). Hens in a positive control group were immunised with human IgG in saline emulsified with an equal volume of Freund's Incomplete Adjuvant. High titres of immunospecific IgY antibodies against human IgG were recorded in the eggs from the chickens immunised orally with the antigen combined with glutaraldehyde conjugated CTB and in the chickens immunised with the antigen combined with Softigen®. The present results show that invasive technique related stress could be eliminated/reduced in polyclonal antibody producing animals.

AB - Oral immunisation by gavage of laying hens with human immunoglobulin G (IgG) combined with a number of potential adjuvants was performed. The resulting immuno-specific egg yolk (IgY) antibodies were quantified by ELISA. The following adjuvants were tested: A Poly(lactide-co-glycolide) (PLG) microspheres, Cholera toxin B-subunit (CTB), CTB conjugated with glutaraldehyde, Dimethyl dioctadecyl ammonium bromide (DDA), and Softigen® (pegylated C8/C10 mono/di glyceride). Hens in a positive control group were immunised with human IgG in saline emulsified with an equal volume of Freund's Incomplete Adjuvant. High titres of immunospecific IgY antibodies against human IgG were recorded in the eggs from the chickens immunised orally with the antigen combined with glutaraldehyde conjugated CTB and in the chickens immunised with the antigen combined with Softigen®. The present results show that invasive technique related stress could be eliminated/reduced in polyclonal antibody producing animals.

KW - Animal welfare

KW - Chicken

KW - Cholera toxin

KW - Egg yolk antibody

KW - Hen

KW - IgY

KW - Immunoglobulin Y

KW - Oral immunisation

KW - Pegylated C8/C10 mono/diglyceride

KW - Softigen

UR - http://www.scopus.com/inward/record.url?scp=0034755095&partnerID=8YFLogxK

M3 - Journal article

C2 - 11695233

AN - SCOPUS:0034755095

VL - 15

SP - 381

EP - 384

JO - In Vivo

JF - In Vivo

SN - 0258-851X

IS - 5

ER -

ID: 369371536