TY - CHAP

T1 - Production of polyclonal and monoclonal antibodies

AU - Hendriksen, Coenraad

AU - Hau, Jann

N1 - Publisher Copyright: © 2003 by CRC Press LLC.

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Many breakthroughs in biomedical science have been achieved by the use of polyclonal and monoclonal antibodies. One of the earliest, and probably best-known examples, is the discovery by Behring and Kitasato in the 1890s1 of the therapeutic effects of diphtheria antiserum. The history of monoclonal antibodies, however, is much younger and dates to the pioneering work of Köhler and Milstein in 1975.2 Nowadays, polyclonal antibodies (Pabs) and monoclonal antibodies (Mabs) are indispensable tools in the laboratory. They are used for immunoassays (e.g., as a diagnostic tool), for affinity chromatography, as immunomarkers (e.g., in pathology), and in basic research (e.g., to discover new proteins and to characterize complex antigenic structures). Furthermore, they are of crucial value in the clinic. Although vaccines have replaced most therapeutic polyclonal antisera, some, such as rabies antiserum and snake antivenom are still important in third world countries. Mabs are increasingly being used as carriers in drug-targeted therapy.

AB - Many breakthroughs in biomedical science have been achieved by the use of polyclonal and monoclonal antibodies. One of the earliest, and probably best-known examples, is the discovery by Behring and Kitasato in the 1890s1 of the therapeutic effects of diphtheria antiserum. The history of monoclonal antibodies, however, is much younger and dates to the pioneering work of Köhler and Milstein in 1975.2 Nowadays, polyclonal antibodies (Pabs) and monoclonal antibodies (Mabs) are indispensable tools in the laboratory. They are used for immunoassays (e.g., as a diagnostic tool), for affinity chromatography, as immunomarkers (e.g., in pathology), and in basic research (e.g., to discover new proteins and to characterize complex antigenic structures). Furthermore, they are of crucial value in the clinic. Although vaccines have replaced most therapeutic polyclonal antisera, some, such as rabies antiserum and snake antivenom are still important in third world countries. Mabs are increasingly being used as carriers in drug-targeted therapy.

UR - http://www.scopus.com/inward/record.url?scp=33750338624&partnerID=8YFLogxK

M3 - Book chapter

AN - SCOPUS:33750338624

SN - 0849310865

SN - 9780849310867

VL - 1

SP - 391

EP - 411

BT - Handbook of Laboratory Animal Science, Second Edition

PB - CRC Press

ER -